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194 macsquantify software version 2 5  (Miltenyi Biotec)


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    Miltenyi Biotec 194 macsquantify software version 2 5
    194 Macsquantify Software Version 2 5, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/macsquantify+software+version+2+5/MACSQuantify+Software/pm39725293-83-12-17
    Average 96 stars, based on 486 article reviews
    194 macsquantify software version 2 5 - by Bioz Stars, 2026-09
    96/100 stars

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    Fluorescence:

    Article Title: Concurrent induction of apoptosis and necroptosis in apigenin‑treated malignant mesothelioma cells: Reversal of Warburg effect through Akt inhibition and p53 upregulation.
    Article Snippet: Cells were treated with or without 20 μM Ly294002 for 2 h, then 30 μM apigenin was added to each well without removing Ly294002 and incubated for 48 h. After trypsin‐ ization, cells were harvested by centrifugation at 500 x g for 7 min, and then resuspended in serum‐free RPMI‐1640 medium containing 2',7'‐dichlorodihydrofluorescein diacetate (10 μM) and rhodamine 123 (30 nM; both from Sigma‐Aldrich; Merck KGaA) in the dark at 37 ̊C for 30 min to measure the levels of ROS and mitochondrial membrane potential, respec‐ tively. .. The fluorescence intensity of the cells was measured with a MACSQuant analyzer and MACSQuantify software version 2.5 (Miltenyi Biotec GmbH). ..

    Article Title: Curcumin and Its Potential to Target the Glycolytic Behavior of Lactate-Acclimated Prostate Carcinoma Cells with Docetaxel
    Article Snippet: .. The fluorescence intensity of the cells was measured using a MACSQuant analyzer and MACSQuantify software version 2.5 (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). ..

    Article Title: Concurrent induction of apoptosis and necroptosis in apigenin‑treated malignant mesothelioma cells: Reversal of Warburg effect through Akt inhibition and p53 upregulation
    Article Snippet: Cells were treated with or without 20 μM Ly294002 for 2 h, then 30 μM apigenin was added to each well without removing Ly294002 and incubated for 48 h. After trypsinization, cells were harvested by centrifugation at 500 × g for 7 min, and then resuspended in serum-free RPMI-1640 medium containing 2′,7′-dichlorodihydrofluorescein diacetate (10 μM) and rhodamine 123 (30 nM; both from Sigma-Aldrich; Merck KGaA) in the dark at 37°C for 30 min to measure the levels of ROS and mitochondrial membrane potential, respectively. .. The fluorescence intensity of the cells was measured with a MACSQuant analyzer and MACSQuantify software version 2.5 (Miltenyi Biotec GmbH). .. Analysis of apoptotic and necrotic cell distribution was performed according to the instructions provided with the Muse Annexin V & Dead Cell Assay Kit (cat. no. MCH100105; Merck KGaA).

    Software:

    Article Title: Concurrent induction of apoptosis and necroptosis in apigenin‑treated malignant mesothelioma cells: Reversal of Warburg effect through Akt inhibition and p53 upregulation.
    Article Snippet: Cells were treated with or without 20 μM Ly294002 for 2 h, then 30 μM apigenin was added to each well without removing Ly294002 and incubated for 48 h. After trypsin‐ ization, cells were harvested by centrifugation at 500 x g for 7 min, and then resuspended in serum‐free RPMI‐1640 medium containing 2',7'‐dichlorodihydrofluorescein diacetate (10 μM) and rhodamine 123 (30 nM; both from Sigma‐Aldrich; Merck KGaA) in the dark at 37 ̊C for 30 min to measure the levels of ROS and mitochondrial membrane potential, respec‐ tively. .. The fluorescence intensity of the cells was measured with a MACSQuant analyzer and MACSQuantify software version 2.5 (Miltenyi Biotec GmbH). ..

    Article Title: Curcumin and Its Potential to Target the Glycolytic Behavior of Lactate-Acclimated Prostate Carcinoma Cells with Docetaxel
    Article Snippet: .. The fluorescence intensity of the cells was measured using a MACSQuant analyzer and MACSQuantify software version 2.5 (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). ..

    Article Title: Concurrent induction of apoptosis and necroptosis in apigenin‑treated malignant mesothelioma cells: Reversal of Warburg effect through Akt inhibition and p53 upregulation.
    Article Snippet: .. After washing the cells with 1X phosphate‐buffered saline (PBS), Muse cell cycle reagent (cat. no. MCH100106; Merck KGaA) containing PI and RNAse was added and allowed to react for 30 min. Data from 10,000 cells were analyzed using the MACSQuant analyzer and MACSQuantify software version 2.5 (MiltenyiBiotec GmbH). ..

    Article Title: Curcumin and Its Potential to Target the Glycolytic Behavior of Lactate-Acclimated Prostate Carcinoma Cells with Docetaxel
    Article Snippet: Cells (1 × 10 6 ) were washed with 1× phosphate-buffered saline (PBS) and incubated with 200 μL of Muse Cell Cycle Reagent (cat. no. MCH100106; Merck KGaA) containing PI and RNase for 30 min at room temperature in the dark. .. Data from 10,000 cells were analyzed using MACSQuant analyzer and MACSQuantify software version 2.5 (Miltenyi Biotec GmbH). .. RNA interference of HK2 was performed using a HK2-targeting small interfering RNA (siHK2) duplex from Invitrogen (Oligo ID, HSS179239).

    Article Title: Concurrent induction of apoptosis and necroptosis in apigenin‑treated malignant mesothelioma cells: Reversal of Warburg effect through Akt inhibition and p53 upregulation
    Article Snippet: Cells were treated with or without 20 μM Ly294002 for 2 h, then 30 μM apigenin was added to each well without removing Ly294002 and incubated for 48 h. After trypsinization, cells were harvested by centrifugation at 500 × g for 7 min, and then resuspended in serum-free RPMI-1640 medium containing 2′,7′-dichlorodihydrofluorescein diacetate (10 μM) and rhodamine 123 (30 nM; both from Sigma-Aldrich; Merck KGaA) in the dark at 37°C for 30 min to measure the levels of ROS and mitochondrial membrane potential, respectively. .. The fluorescence intensity of the cells was measured with a MACSQuant analyzer and MACSQuantify software version 2.5 (Miltenyi Biotec GmbH). .. Analysis of apoptotic and necrotic cell distribution was performed according to the instructions provided with the Muse Annexin V & Dead Cell Assay Kit (cat. no. MCH100105; Merck KGaA).

    Article Title: Concurrent induction of apoptosis and necroptosis in apigenin‑treated malignant mesothelioma cells: Reversal of Warburg effect through Akt inhibition and p53 upregulation
    Article Snippet: .. After washing the cells with 1X phosphate-buffered saline (PBS), Muse cell cycle reagent (cat. no. MCH100106; Merck KGaA) containing PI and RNAse was added and allowed to react for 30 min. Data from 10,000 cells were analyzed using the MACSQuant analyzer and MACSQuantify software version 2.5 (MiltenyiBiotec GmbH). ..

    Article Title: Necroptosis-related lncRNAs signature predicts prognosis and influences immune microenvironment in patients with colon adenocarcinoma
    Article Snippet: .. 5.10 Measurement of reactive oxygen species level and ATP content To determine the reactive oxygen species (ROS) level, CRC cells were seeded on 6-well culture plates at the density of 105 cells per well 24 h prior to treatment with siAP003555.1 or siAC083967.1 in lactic acidcontaining DMEM for 48 h. Following trypsinization, the cells were harvested by centrifugation at 500 × g for 7 min and then resuspended in serum-free DMEM containing 10 μM 2′,7′-dichlorodihydro uorescein Page 15/36 diacetate (DCFH-DA; 5 mM, Sigma–Aldrich, USA) to measure the ROS levels in HT29 and DLD-1 cells in the dark at 37°C for 30 min. After washing the cells twice with 1× PBS, the average uorescence intensity of 10,000 cells was measured using the MACSQuant analyzer and MACSQuantify software version 2.5 (Miltenyi Biotec GmbH). .. Cellular ATP levels were measured using the CellTiter-Glo Luminescent Cell Viability Assay kit (Promega).

    Saline:

    Article Title: Concurrent induction of apoptosis and necroptosis in apigenin‑treated malignant mesothelioma cells: Reversal of Warburg effect through Akt inhibition and p53 upregulation.
    Article Snippet: .. After washing the cells with 1X phosphate‐buffered saline (PBS), Muse cell cycle reagent (cat. no. MCH100106; Merck KGaA) containing PI and RNAse was added and allowed to react for 30 min. Data from 10,000 cells were analyzed using the MACSQuant analyzer and MACSQuantify software version 2.5 (MiltenyiBiotec GmbH). ..

    Article Title: Concurrent induction of apoptosis and necroptosis in apigenin‑treated malignant mesothelioma cells: Reversal of Warburg effect through Akt inhibition and p53 upregulation
    Article Snippet: .. After washing the cells with 1X phosphate-buffered saline (PBS), Muse cell cycle reagent (cat. no. MCH100106; Merck KGaA) containing PI and RNAse was added and allowed to react for 30 min. Data from 10,000 cells were analyzed using the MACSQuant analyzer and MACSQuantify software version 2.5 (MiltenyiBiotec GmbH). ..

    Centrifugation:

    Article Title: Necroptosis-related lncRNAs signature predicts prognosis and influences immune microenvironment in patients with colon adenocarcinoma
    Article Snippet: .. 5.10 Measurement of reactive oxygen species level and ATP content To determine the reactive oxygen species (ROS) level, CRC cells were seeded on 6-well culture plates at the density of 105 cells per well 24 h prior to treatment with siAP003555.1 or siAC083967.1 in lactic acidcontaining DMEM for 48 h. Following trypsinization, the cells were harvested by centrifugation at 500 × g for 7 min and then resuspended in serum-free DMEM containing 10 μM 2′,7′-dichlorodihydro uorescein Page 15/36 diacetate (DCFH-DA; 5 mM, Sigma–Aldrich, USA) to measure the ROS levels in HT29 and DLD-1 cells in the dark at 37°C for 30 min. After washing the cells twice with 1× PBS, the average uorescence intensity of 10,000 cells was measured using the MACSQuant analyzer and MACSQuantify software version 2.5 (Miltenyi Biotec GmbH). .. Cellular ATP levels were measured using the CellTiter-Glo Luminescent Cell Viability Assay kit (Promega).



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